Specific IgE responses in patients allergic to goat’s milk but tolerant to cow’s milk: involvement of minor differences in primary structure between caprine and bovine caseins
نویسندگان
چکیده
Methods b-Lactoglobulin, whole casein and its four different fractions, i.e. as1-, as2-, band -caseins, were isolated from raw CM and GM. Purified b-caseins were subjected to a mild proteolysis by plasmin which generated 3 peptides, i.e. f(1-28), f(29-107) and f(108-207/9). Synthetic peptides partially recovering the N-terminal f(29107) part of the caprine b-casein were also produced. Immunoreactivity of the purified proteins and peptides was assessed by IgE binding studies using sera from 12 GM-allergic patients tolerant to CM and 10 CM-allergic patients. The capacity of bovine and caprine milk proteins to induce the degranulation of humanized rat mast cells passively sensitized with human specific IgE was also evaluated.
منابع مشابه
Mutational analysis of immunodominant epitopes of caprine β-casein recognized by IgE antibodies from patients allergic to goat’s milk and tolerant to cow’s milk
Background Several cases of allergy to goat’s milk (GM) without allergy to cow’s milk (CM) have been reported. GM-allergy has also been reported in CM-allergic children successfully treated with oral immunotherapy. We previously demonstrated that IgE antibodies from GM-allergic/CM-tolerant patients recognize the caprine b-casein (bcap) without cross-reacting with the bovine b-casein (bbov) desp...
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In Europe, a new population of patients developing anaphylaxis to goat’s/sheep’s milk associated to tolerance to cow’s milk has recently appeared. Nevertheless, among these patients, some show allergic reactions to cow’s milk cheeses but these reactions are less severe than those directed against goat’s/sheep’s milk. The objective of the study was to the molecular determinants of the reactivity...
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Cross reactivity between mammalian proteins (cow, goat, ewe, buffalo) has been previously described in both in vitro and in vivo studies. The highest homologies are observed between the milk proteins from cows and other Bovidae, being on average 96.1% for buffalo, 91.1% for ewe, and 87.6% for goat. Lower sequence similarities are associated with proteins contained in milks from Suidae (pigs and...
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Methods 24 children aged between 4 and 13 who achieved tolerance to milk after CM immunotherapy were included. Specific IgE to cow’s caseine, sIgE to whole goat’s milk and sIgE to whole sheep’s milk at baseline and at 24 months follow up were analised (CAP-Phadia). The patientes were classified in two groups: high risk (17 patients) and low risk (7 patients) based on the premedication need, num...
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